7W2A
gliadinase Bga1903
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSRRC BEAMLINE BL15A1 |
| Synchrotron site | NSRRC |
| Beamline | BL15A1 |
| Temperature [K] | 298 |
| Detector technology | CCD |
| Collection date | 2020-02-24 |
| Detector | RAYONIX MX300HE |
| Wavelength(s) | 1 |
| Spacegroup name | P 32 |
| Unit cell lengths | 83.885, 83.885, 107.079 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 26.600 - 1.600 |
| R-factor | 0.1473 |
| Rwork | 0.147 |
| R-free | 0.16970 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 5yl7 |
| Data scaling software | HKL-2000 |
| Phasing software | PHENIX |
| Refinement software | PHENIX (1.17.1_3660) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 30.000 | 30.000 | 1.660 |
| High resolution limit [Å] | 1.600 | 3.450 | 1.600 |
| Rmerge | 0.090 | 0.053 | 0.308 |
| Rmeas | 0.099 | 0.059 | 0.339 |
| Rpim | 0.042 | 0.025 | 0.142 |
| Total number of observations | 620583 | ||
| Number of reflections | 111122 | 11124 | 11117 |
| <I/σ(I)> | 10.4 | ||
| Completeness [%] | 100.0 | 99.9 | 100 |
| Redundancy | 5.6 | 5.5 | 5.6 |
| CC(1/2) | 0.997 | 0.943 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 295.15 | 14.4% (V/V) PEG8000, 80 mM C2H6AsNaO2/HCl (PH6.5), 160 mM Calcium acetate, 20% (V/V) Glycerol |
