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7W1Q

The structure of the Arabidopsis thaliana guanosine deaminase mutant E82Q complexed with 2'-O-methylguanosine

Experimental procedure
Experimental methodSINGLE WAVELENGTH
Source typeROTATING ANODE
Source detailsRIGAKU
Temperature [K]100
Detector technologyPIXEL
Collection date2021-03-17
DetectorDECTRIS PILATUS3 S 6M
Wavelength(s)1.54
Spacegroup nameP 61
Unit cell lengths119.244, 119.244, 39.010
Unit cell angles90.00, 90.00, 120.00
Refinement procedure
Resolution23.690 - 2.300
R-factor0.2125
Rwork0.211
R-free0.23820
Structure solution methodMOLECULAR REPLACEMENT
Starting model (for MR)7dbf
RMSD bond length0.006
RMSD bond angle0.882
Data reduction softwareHKL-3000
Data scaling softwareHKL-3000
Phasing softwarePHENIX
Refinement softwarePHENIX (1.14_3260)
Data quality characteristics
 OverallOuter shell
Low resolution limit [Å]23.6902.420
High resolution limit [Å]2.3002.300
Rmerge0.5151.937
Number of reflections143692074
<I/σ(I)>51.1
Completeness [%]99.9100
Redundancy9.17.5
CC(1/2)0.9540.360
Crystallization Conditions
crystal IDmethodpHtemperaturedetails
1VAPOR DIFFUSION, SITTING DROP7.52771.2 M Na-citrate and 0.1 M HEPES (pH 7.5)

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