7W1Q
The structure of the Arabidopsis thaliana guanosine deaminase mutant E82Q complexed with 2'-O-methylguanosine
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2021-03-17 |
| Detector | DECTRIS PILATUS3 S 6M |
| Wavelength(s) | 1.54 |
| Spacegroup name | P 61 |
| Unit cell lengths | 119.244, 119.244, 39.010 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 23.690 - 2.300 |
| R-factor | 0.2125 |
| Rwork | 0.211 |
| R-free | 0.23820 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 7dbf |
| RMSD bond length | 0.006 |
| RMSD bond angle | 0.882 |
| Data reduction software | HKL-3000 |
| Data scaling software | HKL-3000 |
| Phasing software | PHENIX |
| Refinement software | PHENIX (1.14_3260) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 23.690 | 2.420 |
| High resolution limit [Å] | 2.300 | 2.300 |
| Rmerge | 0.515 | 1.937 |
| Number of reflections | 14369 | 2074 |
| <I/σ(I)> | 5 | 1.1 |
| Completeness [%] | 99.9 | 100 |
| Redundancy | 9.1 | 7.5 |
| CC(1/2) | 0.954 | 0.360 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7.5 | 277 | 1.2 M Na-citrate and 0.1 M HEPES (pH 7.5) |
