7W1H

glycosyltransferase

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL19U1
Synchrotron site	SSRF
Beamline	BL19U1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2021-05-26
Detector	DECTRIS PILATUS3 6M
Wavelength(s)	0.979150
Spacegroup name	P 1 21 1
Unit cell lengths	50.959, 76.120, 63.518
Unit cell angles	90.00, 97.04, 90.00

Refinement procedure

Resolution	38.060 - 2.150
R-factor	0.2009
Rwork	0.199
R-free	0.24760
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	6l8z
Data reduction software	XDS
Data scaling software	Aimless
Phasing software	PHENIX
Refinement software	PHENIX (1.19.2_4158)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	48.550	2.220
High resolution limit [Å]	2.150	2.150
Rmerge	0.146	0.819
Number of reflections	26031	2289
<I/σ(I)>	9.6
Completeness [%]	99.0
Redundancy	6.8

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		291.15	PEG 3350, SODIUM CHLORIDE, MES