7W1B
Glycosyltransferase
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRF BEAMLINE BL19U1 |
| Synchrotron site | SSRF |
| Beamline | BL19U1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2021-05-26 |
| Detector | DECTRIS PILATUS3 6M |
| Wavelength(s) | 0.97915 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 53.351, 74.141, 63.588 |
| Unit cell angles | 90.00, 95.76, 90.00 |
Refinement procedure
| Resolution | 38.790 - 2.150 |
| R-factor | 0.1813 |
| Rwork | 0.179 |
| R-free | 0.22060 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 6l8z |
| RMSD bond length | 0.007 |
| RMSD bond angle | 0.855 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | PHENIX |
| Refinement software | PHENIX (1.19.2_4158) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 43.160 | 2.220 |
| High resolution limit [Å] | 2.150 | 2.150 |
| Number of reflections | 26886 | 2333 |
| <I/σ(I)> | 12.9 | |
| Completeness [%] | 99.7 | |
| Redundancy | 6.6 | |
| CC(1/2) | 0.975 | 0.862 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 291.15 | PEG 3350, Sodium chloride, MES |
