7W0Z

Glycosyltranferase UGT74AN2

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL19U1
Synchrotron site	SSRF
Beamline	BL19U1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2021-05-26
Detector	DECTRIS PILATUS3 6M
Wavelength(s)	0.97915
Spacegroup name	P 1 21 1
Unit cell lengths	51.026, 76.553, 63.553
Unit cell angles	90.00, 96.92, 90.00

Refinement procedure

Resolution	42.240 - 2.100
R-factor	0.1905
Rwork	0.188
R-free	0.22950
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	6l8z
Data reduction software	XDS
Data scaling software	Aimless
Phasing software	PHENIX
Refinement software	PHENIX (1.19.2_4158)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	48.690	1.940
High resolution limit [Å]	1.900	1.900
Number of reflections	38306	2415
<I/σ(I)>	11.5
Completeness [%]	99.9
Redundancy	17.7
CC(1/2)	0.956	0.733

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		291.15	PEG 3350, 0.1M sodium chloride, 0.1M MES (pH 6.5)