7W0W
The novel membrane-proximal sensing mechanism in a broad-ligand binding chemoreceptor McpA of Bacillus velezensis
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SSRF BEAMLINE BL17U1 |
Synchrotron site | SSRF |
Beamline | BL17U1 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2020-05-31 |
Detector | DECTRIS EIGER X 16M |
Wavelength(s) | 0.97918 |
Spacegroup name | P 21 21 2 |
Unit cell lengths | 180.161, 32.855, 57.903 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 48.756 - 2.247 |
Rwork | 0.182 |
R-free | 0.23670 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 6pxy |
RMSD bond length | 0.009 |
RMSD bond angle | 1.470 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | PHASER |
Refinement software | REFMAC (5.8.0267) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 2.290 |
High resolution limit [Å] | 2.247 | 2.250 |
Rmerge | 0.091 | 0.477 |
Number of reflections | 17195 | 821 |
<I/σ(I)> | 20.4 | |
Completeness [%] | 99.7 | |
Redundancy | 7.8 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 293 | 0.17 M ammonium acetate, 0.085M sodium acetate pH 4.6, 25.5 % PEG 4000, 15 % glycerol |