7VZO

crystal structure of Domain 5-6 of filamin C from Scylla paramamosain

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL02U1
Synchrotron site	SSRF
Beamline	BL02U1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2021-07-07
Detector	DECTRIS EIGER2 X 9M
Wavelength(s)	0.97918
Spacegroup name	P 1 21 1
Unit cell lengths	51.604, 70.723, 59.012
Unit cell angles	90.00, 113.19, 90.00

Refinement procedure

Resolution	24.220 - 1.750
R-factor	0.1634
Rwork	0.161
R-free	0.20450
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	2j3s
RMSD bond length	0.012
RMSD bond angle	1.735
Data reduction software	XDS
Data scaling software	Aimless (0.7.4)
Phasing software	MOLREP
Refinement software	REFMAC (5.8.0267)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	24.220	1.780
High resolution limit [Å]	1.750	1.750
Rmerge	0.045	0.240
Rmeas	0.064	0.339
Rpim	0.045	0.240
Total number of observations	77080
Number of reflections	39401	2184
<I/σ(I)>	8.5
Completeness [%]	100.0	100
Redundancy	2	2
CC(1/2)	0.986	0.636

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7.8	298	0.2 M ammonium acetate 0.1 M HEPES pH 7.8 20%(V/V) PEG 6000