7VY7

Snapshots of Human PSMD10(Gankyrin) unfolding by urea: 3 hours incubation

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	BRUKER AXS MICROSTAR
Temperature [K]	100
Detector technology	IMAGE PLATE
Collection date	2021-03-09
Detector	MAR scanner 345 mm plate
Wavelength(s)	1.5418
Spacegroup name	P 32 2 1
Unit cell lengths	60.140, 60.140, 122.430
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	52.080 - 2.230
R-factor	0.2469
Rwork	0.240
R-free	0.31310
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1uoh
RMSD bond length	0.007
RMSD bond angle	1.616
Data reduction software	MOSFLM
Data scaling software	SCALA (3.3.22)
Phasing software	PHASER
Refinement software	REFMAC (5.8.0267)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	52.083	52.083	2.330
High resolution limit [Å]	2.228	7.000	2.228
Rmerge		0.039	1.384
Rmeas	0.281	0.042	1.477
Rpim	0.096	0.015	0.508
Total number of observations	109128
Number of reflections	13085	501	1602
<I/σ(I)>	7.4	15.4	0.6
Completeness [%]	97.6	99.9	83.7
Redundancy	8.3	7.4	8.1

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	7	295	60% Tacsimate pH 7