7VY4
Snapshots of Human PSMD10(Gankyrin) unfolding by urea: 2 hours incubation
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | ROTATING ANODE |
Source details | BRUKER AXS MICROSTAR |
Temperature [K] | 100 |
Detector technology | IMAGE PLATE |
Collection date | 2021-03-10 |
Detector | MAR scanner 345 mm plate |
Wavelength(s) | 1.5418 |
Spacegroup name | P 32 2 1 |
Unit cell lengths | 60.300, 60.300, 122.310 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 52.220 - 2.220 |
R-factor | 0.2475 |
Rwork | 0.241 |
R-free | 0.31210 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1uoh |
RMSD bond length | 0.007 |
RMSD bond angle | 1.670 |
Data reduction software | MOSFLM |
Data scaling software | SCALA (3.3.22) |
Phasing software | PHASER |
Refinement software | REFMAC (5.8.0267) |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 52.221 | 52.221 | 2.330 |
High resolution limit [Å] | 2.220 | 7.000 | 2.224 |
Rmerge | 0.033 | 1.702 | |
Rmeas | 0.295 | 0.035 | 1.813 |
Rpim | 0.099 | 0.012 | 0.614 |
Number of reflections | 13221 | 502 | 1688 |
<I/σ(I)> | 7.5 | 20.5 | 0.5 |
Completeness [%] | 98.2 | 99.8 | 88.1 |
Redundancy | 8.6 | 7.7 | 8.2 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 7 | 295 | 60% Tacsimate pH7 |