7VXV
Snapshots of Human PSMD10(Gankyrin) unfolding by urea: 0 hours incubation / Native
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | BRUKER AXS MICROSTAR |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2021-03-08 |
| Detector | MAR scanner 345 mm plate |
| Wavelength(s) | 1.5418 |
| Spacegroup name | P 32 2 1 |
| Unit cell lengths | 59.990, 59.990, 122.310 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 51.950 - 2.230 |
| R-factor | 0.201 |
| Rwork | 0.196 |
| R-free | 0.24350 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1uoh |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.521 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA (3.3.22) |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0267) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 51.953 | 51.953 | 2.330 |
| High resolution limit [Å] | 2.226 | 7.000 | 2.226 |
| Rmerge | 0.025 | 0.567 | |
| Rmeas | 0.134 | 0.027 | 0.602 |
| Rpim | 0.044 | 0.009 | 0.199 |
| Total number of observations | 113463 | ||
| Number of reflections | 12766 | 485 | 1580 |
| <I/σ(I)> | 16 | 25.7 | 1.3 |
| Completeness [%] | 96.5 | 99.3 | 83.9 |
| Redundancy | 8.9 | 7.9 | 8.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7 | 295 | 60% Tacsimate pH 7.0 |
