7VXO
Structure of the C-terminal head domain of the Fowl Adenovirus serotype 4 (FAdV-4) fibre-2 protein
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ALS BEAMLINE 4.2.2 |
Synchrotron site | ALS |
Beamline | 4.2.2 |
Temperature [K] | 293 |
Detector technology | CCD |
Collection date | 2021-06-26 |
Detector | ADSC QUANTUM 210r |
Wavelength(s) | 1 |
Spacegroup name | P 3 2 1 |
Unit cell lengths | 60.303, 60.303, 92.095 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 25.240 - 1.600 |
R-factor | 0.14 |
Rwork | 0.138 |
R-free | 0.18200 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 2vtw |
RMSD bond length | 0.014 |
RMSD bond angle | 1.752 |
Data reduction software | HKL-2000 |
Data scaling software | SCALEPACK |
Phasing software | PHASER |
Refinement software | REFMAC (5.8.0230) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 30.000 | 1.660 |
High resolution limit [Å] | 1.600 | 1.600 |
Rmerge | 0.103 | 0.296 |
Rmeas | 0.106 | 0.060 |
Rpim | 0.024 | 0.014 |
Total number of observations | 475715 | |
Number of reflections | 25997 | 2808 |
<I/σ(I)> | 6.7 | |
Completeness [%] | 98.6 | 86.5 |
Redundancy | 18.3 | 14.2 |
CC(1/2) | 0.999 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 293 | 0.2M Ammonium sulfate, 0.1M BIS-TRIS pH6.5, 25% w/v Polyethylene glycol 3350 |