7VWV

The crystal structure of African swine fever virus I73R

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL18U1
Synchrotron site	SSRF
Beamline	BL18U1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2021-09-25
Detector	DECTRIS PILATUS3 6M
Wavelength(s)	0.97915
Spacegroup name	H 3
Unit cell lengths	90.544, 90.544, 49.390
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	26.150 - 2.590
R-factor	0.1908
Rwork	0.187
R-free	0.26040
Structure solution method	SAD
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	AutoSol
Refinement software	PHENIX (1.19)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	50.000	50.000	2.630
High resolution limit [Å]	2.590	7.010	2.590
Rmerge	0.068	0.060	0.166
Rmeas	0.071	0.063	0.177
Rpim	0.022	0.020	0.061
Total number of observations	43712
Number of reflections	4600	234	183
<I/σ(I)>	11.4
Completeness [%]	97.6	100	78.5
Redundancy	9.5	9.6	6.9
CC(1/2)		0.998	0.974

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		293	0.2M kalium chloratum, 16% PEG 3350