7VWT

Carbazole Prenyl Transferase CqsB4

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SPRING-8 BEAMLINE BL32XU
Synchrotron site	SPring-8
Beamline	BL32XU
Temperature [K]	93.15
Detector technology	PIXEL
Collection date	2021-10-05
Detector	DECTRIS EIGER X 9M
Wavelength(s)	0.999999
Spacegroup name	P 21 21 21
Unit cell lengths	73.261, 85.251, 113.405
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	44.840 - 1.730
R-factor	0.1998
Rwork	0.198
R-free	0.23540
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	Alpha Fold2 model
RMSD bond length	0.010
RMSD bond angle	1.027
Data reduction software	XDS
Data scaling software	XDS
Phasing software	PHASER
Refinement software	PHENIX (1.18.2_3874)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	44.840	1.792
High resolution limit [Å]	1.730	1.730
Number of reflections	74583	14623
<I/σ(I)>	12.43
Completeness [%]	99.8
Redundancy	2
CC(1/2)	0.999	0.603

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION		277.15	LiSO4, PEG 3350