7VWB
Phloem lectin (PP2) structure -complex with N-Acetyllactosamine (LacNAc)
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | ROTATING ANODE |
Source details | RIGAKU ULTRAX 18 |
Temperature [K] | 100 |
Detector technology | IMAGE PLATE |
Collection date | 2020-02-12 |
Detector | RIGAKU RAXIS IV++ |
Wavelength(s) | 1.5418 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 37.690, 60.390, 82.980 |
Unit cell angles | 90.00, 99.13, 90.00 |
Refinement procedure
Resolution | 24.896 - 1.900 |
Rwork | 0.218 |
R-free | 0.24850 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 7vs6 |
RMSD bond length | 0.010 |
RMSD bond angle | 1.567 |
Data reduction software | iMOSFLM |
Data scaling software | SCALA |
Phasing software | PHASER |
Refinement software | REFMAC (5.8.0258) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 30.190 | 2.000 |
High resolution limit [Å] | 1.900 | 1.900 |
Rmerge | 0.130 | 0.180 |
Number of reflections | 28551 | 4055 |
<I/σ(I)> | 7 | |
Completeness [%] | 98.0 | |
Redundancy | 4 | |
CC(1/2) | 0.970 | 0.880 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | MICROBATCH | 295 | 0.1 M MES monohydrate pH 6.0, 22% v/v Polyethylene glycol 400 |