7VW8
Helicoverpa armigera pheromone-binding protein PBP1 at pH 7.5
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRF BEAMLINE BL17U1 |
| Synchrotron site | SSRF |
| Beamline | BL17U1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2014-05-12 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 0.9792 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 32.030, 32.604, 54.792 |
| Unit cell angles | 90.00, 97.88, 90.00 |
Refinement procedure
| Resolution | 20.866 - 1.300 |
| R-factor | 0.1797 |
| Rwork | 0.179 |
| R-free | 0.19760 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2fjy |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.378 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | BALBES |
| Refinement software | REFMAC (5.8.0267) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 1.320 |
| High resolution limit [Å] | 1.300 | 1.300 |
| Rmerge | 0.066 | 0.500 |
| Number of reflections | 27772 | 1306 |
| <I/σ(I)> | 17.7 | |
| Completeness [%] | 93.1 | |
| Redundancy | 4.3 | |
| CC(1/2) | 0.985 | 0.912 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 298 | peg |
