7VW2
Structure of a dimeric periplasmic protein bound with cupric ions
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRF BEAMLINE BL19U1 |
| Synchrotron site | SSRF |
| Beamline | BL19U1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2021-10-01 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 0.9792 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 50.446, 74.412, 50.786 |
| Unit cell angles | 90.00, 96.18, 90.00 |
Refinement procedure
| Resolution | 33.607 - 2.186 |
| R-factor | 0.2472 |
| Rwork | 0.245 |
| R-free | 0.28180 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 5ffb |
| RMSD bond length | 0.005 |
| RMSD bond angle | 0.662 |
| Data reduction software | HKL-3000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.12_2829) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.270 |
| High resolution limit [Å] | 2.186 | 2.200 |
| Rmerge | 0.083 | 0.542 |
| Rpim | 0.037 | 0.237 |
| Number of reflections | 9492 | 941 |
| <I/σ(I)> | 20.8 | 3.5 |
| Completeness [%] | 97.9 | 98.1 |
| Redundancy | 6.2 | 6.1 |
| CC(1/2) | 0.996 | 0.932 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 289 | Sodium thiocyanate, AmSO4 |
