7VW1

Structure of a dimeric periplasmic protein bound with cuprous ions

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL19U1
Synchrotron site	SSRF
Beamline	BL19U1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2021-10-01
Detector	DECTRIS PILATUS 6M
Wavelength(s)	0.9792
Spacegroup name	P 43
Unit cell lengths	77.415, 77.415, 44.933
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	34.621 - 2.494
R-factor	0.2052
Rwork	0.203
R-free	0.24840
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	5ffb
RMSD bond length	0.004
RMSD bond angle	0.681
Data reduction software	HKL-3000
Data scaling software	HKL-2000
Phasing software	PHASER
Refinement software	PHENIX (1.12_2829)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	2.590
High resolution limit [Å]	2.494	2.494
Rmerge	0.132	0.905
Rpim	0.049	0.309
Number of reflections	9526	930
<I/σ(I)>	19.3
Completeness [%]	99.9
Redundancy	19.3
CC(1/2)	0.983	0.956

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		289	Lithium chloride, AmSO4