7VW0

Structure of a dimeric periplasmic protein

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL19U1
Synchrotron site	SSRF
Beamline	BL19U1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2021-05-16
Detector	DECTRIS PILATUS 6M
Wavelength(s)	0.9792
Spacegroup name	P 21 21 21
Unit cell lengths	37.296, 73.242, 73.284
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	20.435 - 1.447
R-factor	0.1565
Rwork	0.154
R-free	0.19680
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	5ffb
RMSD bond length	0.007
RMSD bond angle	0.821
Data reduction software	HKL-3000
Data scaling software	HKL-2000
Phasing software	PHASER
Refinement software	PHENIX (1.12_2829)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	1.500
High resolution limit [Å]	1.447	1.450
Rmerge	0.113
Rpim	0.035	0.328
Number of reflections	35568	3440
<I/σ(I)>	21.5
Completeness [%]	97.3
Redundancy	12.5
CC(1/2)	0.997	0.870

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		289	MES, Ammonium sulfate