7VVW
MmtN-SAM complex
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRF BEAMLINE BL17U1 |
| Synchrotron site | SSRF |
| Beamline | BL17U1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2019-10-21 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 0.97915 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 62.017, 129.308, 134.248 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 93.130 - 2.110 |
| R-factor | 0.2033 |
| Rwork | 0.202 |
| R-free | 0.23350 |
| Structure solution method | SAD |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | AutoSol |
| Refinement software | PHENIX (1.17.1_3660) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 93.130 | 2.190 |
| High resolution limit [Å] | 2.110 | 2.110 |
| Rmerge | 0.115 | 1.205 |
| Rmeas | 0.119 | 1.252 |
| Rpim | 0.034 | 0.337 |
| Number of reflections | 61893 | 6159 |
| <I/σ(I)> | 12.8 | 2.3 |
| Completeness [%] | 98.6 | 99.94 |
| Redundancy | 12.8 | 13.7 |
| CC(1/2) | 0.998 | 0.911 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 291.15 | 0.2M sodium acetate trihydrate, 0.1M sodium cacodylate trihydrate pH 6.5, 15% (wt/vol) PEG 8000 |
