7VV8

Crystal Structure of HRasQ61L(GMPPNP-bound) in complex with the Ras-binding domain(RBD) of SIN1

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	NFPSS BEAMLINE BL19U1
Synchrotron site	NFPSS
Beamline	BL19U1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2019-06-07
Detector	DECTRIS PILATUS 6M
Wavelength(s)	0.979
Spacegroup name	P 61 2 2
Unit cell lengths	101.316, 101.316, 124.291
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	26.490 - 1.700
Rwork	0.180
R-free	0.19880
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4g0n
Data reduction software	XDS
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	PHENIX (1.20rc1_4392)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	26.490	1.761
High resolution limit [Å]	1.700	1.700
Rmerge	0.076	0.753
Number of reflections	41520	4098
<I/σ(I)>	25.57
Completeness [%]	98.9	99.78
Redundancy	16.5
CC(1/2)	1.000	0.897

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		277	PEG20000, 0.1M Sodim citrate. pH 5.0