7VUP
Structure of NF-kB p52 homodimer bound to +1/-1 swap P-Selectin kB DNA fragment
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SSRF BEAMLINE BL17U1 |
Synchrotron site | SSRF |
Beamline | BL17U1 |
Temperature [K] | 93 |
Detector technology | PIXEL |
Collection date | 2019-11-11 |
Detector | DECTRIS EIGER X 16M |
Wavelength(s) | 0.979183 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 83.987, 84.289, 140.565 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 45.451 - 3.400 |
Rwork | 0.271 |
R-free | 0.28580 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1a3q |
RMSD bond length | 0.010 |
RMSD bond angle | 1.735 |
Data reduction software | XDS |
Data scaling software | SCALA |
Phasing software | PHASER |
Refinement software | REFMAC (5.8.0267) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 46.850 | 3.580 |
High resolution limit [Å] | 3.400 | 3.400 |
Number of reflections | 14272 | 2025 |
<I/σ(I)> | 9.6 | |
Completeness [%] | 99.7 | |
Redundancy | 13 | |
CC(1/2) | 0.998 | 0.782 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 293 | 0.1M Sodium malonate, pH 4.0, 50mM CsCl, 2.5% PEG 3350 |