7VUD

Carotenoid Cleavage Dioxygenase 1 from Osmanthus fragrans

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	AUSTRALIAN SYNCHROTRON BEAMLINE MX1
Synchrotron site	Australian Synchrotron
Beamline	MX1
Temperature [K]	98.15
Detector technology	CCD
Collection date	2021-02-24
Detector	ADSC QUANTUM 210r
Wavelength(s)	0.98
Spacegroup name	P 41 3 2
Unit cell lengths	209.941, 209.941, 209.941
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	49.480 - 2.800
Rwork	0.227
R-free	0.25840
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	3npe
RMSD bond length	0.006
RMSD bond angle	1.451
Data reduction software	XDS
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	REFMAC (5.8.0267)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	49.530	2.910
High resolution limit [Å]	2.800	2.800
Rmerge	0.015	0.320
Number of reflections	39479	4387
<I/σ(I)>	10.8	1.1
Completeness [%]	100.0
Redundancy	81
CC(1/2)	0.996	0.380

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION	7.5	293.15	0.1M Tris pH 7.5, 1.5M Ammonium Sulfate