Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ELETTRA BEAMLINE 11.2C |
| Synchrotron site | ELETTRA |
| Beamline | 11.2C |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2020-03-04 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 1.0 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 42.810, 70.470, 101.950 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 50.900 - 1.200 |
| Rwork | 0.160 |
| R-free | 0.18930 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 7vs6 |
| RMSD bond length | 0.018 |
| RMSD bond angle | 2.026 |
| Data reduction software | iMOSFLM |
| Data scaling software | SCALA |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0258) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.970 | 1.220 |
| High resolution limit [Å] | 1.200 | 1.200 |
| Rmerge | 0.073 | 1.270 |
| Number of reflections | 2585878 | 89698 |
| <I/σ(I)> | 2.5 | |
| Completeness [%] | 100.0 | |
| Redundancy | 26.6 | |
| CC(1/2) | 1.000 | 0.850 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | MICROBATCH | 295 | 1.0 M Ammonium citrate dibasic, 0.1 M Sodium acetate trihydrate pH 4.6 |
