7VUA
Anaerobic hydroxyproline degradation involving C-N cleavage by a glycyl radical enzyme
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SSRF BEAMLINE BL18U1 |
Synchrotron site | SSRF |
Beamline | BL18U1 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2021-01-25 |
Detector | DECTRIS PILATUS3 6M |
Wavelength(s) | 0.9795 |
Spacegroup name | C 2 2 21 |
Unit cell lengths | 117.841, 218.212, 168.174 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 32.142 - 2.695 |
R-factor | 0.176 |
Rwork | 0.174 |
R-free | 0.24320 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | Phyre2 prediction struction |
Data reduction software | HKL-3000 |
Data scaling software | HKL-2000 |
Phasing software | PHENIX |
Refinement software | PHENIX (1.14_3247) |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 50.000 | 50.000 | 2.750 |
High resolution limit [Å] | 2.695 | 7.320 | 2.700 |
Rmerge | 0.141 | 0.051 | 0.939 |
Rmeas | 0.147 | 0.053 | 0.975 |
Rpim | 0.040 | 0.015 | 0.263 |
Number of reflections | 59889 | 3182 | 2931 |
<I/σ(I)> | 3.6 | ||
Completeness [%] | 100.0 | 100 | 100 |
Redundancy | 13.3 | 12.3 | 13.5 |
CC(1/2) | 0.999 | 0.831 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 291.15 | 0.1M HEPES pH 7.5, 10% (w/v) PEG 8000, 8% (v/v) Ethylene glycol |