7VTR

3-ketoacyl-CoA thiolase Tfu_0875

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL17B1
Synchrotron site	SSRF
Beamline	BL17B1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2021-07-11
Detector	DECTRIS PILATUS 6M
Wavelength(s)	0.9793
Spacegroup name	P 21 21 21
Unit cell lengths	154.470, 71.070, 158.648
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	26.680 - 1.930
R-factor	0.1803
Rwork	0.180
R-free	0.21470
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1ulq
Refinement software	PHENIX (v 1.19)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	1.960
High resolution limit [Å]	1.930	1.930
Number of reflections	131106	130937
<I/σ(I)>	15
Completeness [%]	99.9
Redundancy	12.1
CC(1/2)	0.985	0.985

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	6.5	289.15	PEG 3350, Ammonium acetate, BIS-TRIS