7VTK

Crystal structure of Glycoside Hydrolases family 64 beta-1,3-glucanase

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL17U
Synchrotron site	SSRF
Beamline	BL17U
Temperature [K]	100
Detector technology	PIXEL
Collection date	2021-01-05
Detector	DECTRIS EIGER2 X 16M
Wavelength(s)	0.979
Spacegroup name	P 32 2 1
Unit cell lengths	78.989, 78.989, 157.647
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	24.567 - 1.907
R-factor	0.201425243383
Rwork	0.200
R-free	0.24046
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	3gd0
RMSD bond length	0.008
RMSD bond angle	0.947
Data reduction software	HKL-3000
Data scaling software	HKL-3000
Phasing software	PHENIX (dev_2474)
Refinement software	PHENIX (dev_2474)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	24.570	1.975
High resolution limit [Å]	1.900	1.900
Rmerge	0.079
Number of reflections	45108
<I/σ(I)>	43.8
Completeness [%]	99.0
Redundancy	19.4

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		293	1.1M sodium malonate pH 7.0, 0.1M HEPES pH 7.0