7VTK
Crystal structure of Glycoside Hydrolases family 64 beta-1,3-glucanase
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SSRF BEAMLINE BL17U |
Synchrotron site | SSRF |
Beamline | BL17U |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2021-01-05 |
Detector | DECTRIS EIGER2 X 16M |
Wavelength(s) | 0.979 |
Spacegroup name | P 32 2 1 |
Unit cell lengths | 78.989, 78.989, 157.647 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 24.567 - 1.907 |
R-factor | 0.201425243383 |
Rwork | 0.200 |
R-free | 0.24046 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 3gd0 |
RMSD bond length | 0.008 |
RMSD bond angle | 0.947 |
Data reduction software | HKL-3000 |
Data scaling software | HKL-3000 |
Phasing software | PHENIX (dev_2474) |
Refinement software | PHENIX (dev_2474) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 24.570 | 1.975 |
High resolution limit [Å] | 1.900 | 1.900 |
Rmerge | 0.079 | |
Number of reflections | 45108 | |
<I/σ(I)> | 43.8 | |
Completeness [%] | 99.0 | |
Redundancy | 19.4 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 293 | 1.1M sodium malonate pH 7.0, 0.1M HEPES pH 7.0 |