7VTG
Pseudouridine bound structure of Pseudouridine kinase (PUKI) S30A mutant from Escherichia coli strain B
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | PAL/PLS BEAMLINE 7A (6B, 6C1) |
Synchrotron site | PAL/PLS |
Beamline | 7A (6B, 6C1) |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2020-08-05 |
Detector | ADSC QUANTUM 270 |
Wavelength(s) | 0.97932 |
Spacegroup name | P 3 |
Unit cell lengths | 185.092, 185.092, 51.747 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 33.722 - 1.899 |
R-factor | 0.219675766695 |
Rwork | 0.219 |
R-free | 0.26222 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 3kzh |
RMSD bond length | 0.018 |
RMSD bond angle | 1.662 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | PHENIX |
Refinement software | PHENIX (1.9_1692) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 1.970 |
High resolution limit [Å] | 1.890 | 1.900 |
Number of reflections | 155963 | 15713 |
<I/σ(I)> | 15.3 | |
Completeness [%] | 99.6 | |
Redundancy | 5.2 | |
CC(1/2) | 0.985 | 0.427 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 295 | 0.2M magnesium formate, 20%PEG 3350, 5% glycerol |