7VTD
Unliganded structure of Pseudouridine kinase (PUKI) from Escherichia coli strain B
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | PAL/PLS BEAMLINE 7A (6B, 6C1) |
Synchrotron site | PAL/PLS |
Beamline | 7A (6B, 6C1) |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2011-11-11 |
Detector | ADSC QUANTUM 270 |
Wavelength(s) | 0.97934 |
Spacegroup name | P 3 |
Unit cell lengths | 186.805, 186.805, 49.648 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 33.289 - 2.151 |
R-factor | 0.232811756303 |
Rwork | 0.232 |
R-free | 0.26763 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 3kzh |
RMSD bond length | 0.004 |
RMSD bond angle | 0.905 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | PHENIX |
Refinement software | PHENIX (1.9_1692) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 2.230 |
High resolution limit [Å] | 2.150 | 2.150 |
Number of reflections | 104841 | 10500 |
<I/σ(I)> | 15 | |
Completeness [%] | 99.7 | |
Redundancy | 5.4 | |
CC(1/2) | 0.990 | 0.500 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 295 | 0.2M magnesium formate, 20% PEG 3350, 5% glycerol |