7VTA
Talaromyces verruculosus talaropentaene synthase apo
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SLS BEAMLINE X06SA |
Synchrotron site | SLS |
Beamline | X06SA |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2020-09-04 |
Detector | DECTRIS EIGER X 16M |
Wavelength(s) | 1.0 |
Spacegroup name | I 1 2 1 |
Unit cell lengths | 76.503, 61.770, 81.071 |
Unit cell angles | 90.00, 116.28, 90.00 |
Refinement procedure
Resolution | 47.070 - 2.400 |
R-factor | 0.2328 |
Rwork | 0.231 |
R-free | 0.27020 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 5er8 |
RMSD bond length | 0.007 |
RMSD bond angle | 0.911 |
Data reduction software | XDS |
Data scaling software | Aimless |
Phasing software | PHASER |
Refinement software | PHENIX (1.19.2_4158) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 47.070 | 2.490 |
High resolution limit [Å] | 2.400 | 2.400 |
Rmerge | 0.097 | 0.568 |
Number of reflections | 13274 | 9399 |
<I/σ(I)> | 13.3 | 3.5 |
Completeness [%] | 99.2 | |
Redundancy | 6.9 | 6.8 |
CC(1/2) | 0.997 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 8.5 | 293 | 0.1 M Tris-Cl (pH 8.5), 0.1 M MgCl2, 30% PEG 4000, 0.2 M NDSB-211 |