7VT8
Crystal structure of MtGlu5 from Meiothermus taiwanensis WR-220
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | NSRRC BEAMLINE TPS 05A |
Synchrotron site | NSRRC |
Beamline | TPS 05A |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2021-04-11 |
Detector | RAYONIX MX300-HS |
Wavelength(s) | 1.0 |
Spacegroup name | I 4 2 2 |
Unit cell lengths | 144.929, 144.929, 197.568 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 29.210 - 2.990 |
R-factor | 0.2077 |
Rwork | 0.205 |
R-free | 0.24900 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 3mmu |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | MOLREP |
Refinement software | PHENIX (1.17.1_3660) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 3.040 |
High resolution limit [Å] | 2.990 | 2.990 |
Rmerge | 0.071 | |
Rmeas | 0.076 | |
Rpim | 0.027 | 0.439 |
Number of reflections | 21603 | 1055 |
<I/σ(I)> | 26.55 | |
Completeness [%] | 99.8 | |
Redundancy | 7.8 | |
CC(1/2) | 0.866 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | MICROBATCH | 298 | 0.16M citric acid, 1.1M ammonium sulfate, 10% PEG-400, pH 5.0 |