7VT5
Crystal structure of CBM deleted MtGlu5 from Meiothermus taiwanensis WR-220
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | NSRRC BEAMLINE BL15A1 |
Synchrotron site | NSRRC |
Beamline | BL15A1 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2021-03-28 |
Detector | RAYONIX MX300HE |
Wavelength(s) | 1.0 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 49.549, 75.348, 82.151 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 24.220 - 1.460 |
R-factor | 0.1494 |
Rwork | 0.148 |
R-free | 0.17430 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 3mmu |
RMSD bond length | 0.011 |
RMSD bond angle | 1.145 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | MOLREP |
Refinement software | PHENIX (1.17.1_3660) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 1.510 |
High resolution limit [Å] | 1.460 | 1.460 |
Rmerge | 0.057 | 0.503 |
Rmeas | 0.600 | |
Rpim | 0.034 | 0.320 |
Number of reflections | 51808 | 4780 |
<I/σ(I)> | 26.14 | |
Completeness [%] | 96.0 | |
Redundancy | 3.7 | |
CC(1/2) | 0.856 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | EVAPORATION | 298 | 20mM Tris, 100mM NaCl, pH 8.0 |