7VSX
Crystal structure of QL-nanoKAZ (Reverse mutant of nanoKAZ with L18Q and V27L)
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SPRING-8 BEAMLINE BL26B2 |
Synchrotron site | SPring-8 |
Beamline | BL26B2 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2016-11-18 |
Detector | DECTRIS PILATUS 12M |
Wavelength(s) | 1 |
Spacegroup name | I 2 2 2 |
Unit cell lengths | 60.810, 76.000, 103.820 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 47.482 - 1.698 |
R-factor | 0.1836 |
Rwork | 0.182 |
R-free | 0.20660 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 5b0u |
RMSD bond length | 0.014 |
RMSD bond angle | 1.296 |
Data reduction software | XDS |
Data scaling software | XSCALE |
Phasing software | PHASER (2.6.0) |
Refinement software | PHENIX (1.18.2) |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 47.482 | 47.482 | 1.800 |
High resolution limit [Å] | 1.698 | 5.070 | 1.700 |
Rmerge | 0.070 | 0.050 | 0.887 |
Rmeas | 0.076 | 0.054 | 0.956 |
Number of reflections | 26866 | 1103 | 4211 |
<I/σ(I)> | 15.08 | 34.74 | 2.24 |
Completeness [%] | 99.7 | 99.6 | 98.5 |
Redundancy | 7.319 | 6.848 | 7.215 |
CC(1/2) | 0.998 | 0.997 | 0.936 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 6.5 | 293 | 100 mM MES (pH6.5) 1.8-1.9 M MgSO4 |