7VSW
Crystal structure of a Fab-like fragment of anti-mesothelin antibody
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SSRF BEAMLINE BL17U1 |
Synchrotron site | SSRF |
Beamline | BL17U1 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2020-10-09 |
Detector | DECTRIS PILATUS 6M |
Wavelength(s) | 0.9792 |
Spacegroup name | P 43 21 2 |
Unit cell lengths | 143.900, 143.900, 261.890 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 21.170 - 3.000 |
R-factor | 0.2183 |
Rwork | 0.216 |
R-free | 0.25250 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 6cnr |
RMSD bond length | 0.003 |
RMSD bond angle | 0.632 |
Data reduction software | xia2 |
Data scaling software | Aimless |
Phasing software | PHASER |
Refinement software | PHENIX (1.15.2_3472) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 28.220 | 3.080 |
High resolution limit [Å] | 3.000 | 3.000 |
Rmerge | 0.201 | |
Rpim | 0.722 | |
Number of reflections | 55749 | 4039 |
<I/σ(I)> | 17.4 | 2 |
Completeness [%] | 99.9 | |
Redundancy | 26.6 | |
CC(1/2) | 0.999 | 0.750 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 293 | 4.0 M sodium chloride and 0.1 M HEPES, pH 7.0 |