7VSU

Crystal structure of Fab fragment of anti-mesothelin antibody

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL18U1
Synchrotron site	SSRF
Beamline	BL18U1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2021-01-06
Detector	DECTRIS PILATUS 6M
Wavelength(s)	0.9793
Spacegroup name	P 31
Unit cell lengths	131.755, 131.755, 90.713
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	25.950 - 3.100
R-factor	0.2171
Rwork	0.215
R-free	0.24850
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	6cnr
RMSD bond length	0.002
RMSD bond angle	0.592
Data reduction software	HKL-3000
Data scaling software	HKL-3000
Phasing software	PHASER
Refinement software	PHENIX (1.15.2_3472)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	35.180	3.150
High resolution limit [Å]	3.100	3.100
Rmerge	0.199
Rpim	0.066	0.595
Number of reflections	31825	1606
<I/σ(I)>	18.1
Completeness [%]	100.0
Redundancy	10.3
CC(1/2)		0.736

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		293	4.0 M sodium formate