7VSU
Crystal structure of Fab fragment of anti-mesothelin antibody
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRF BEAMLINE BL18U1 |
| Synchrotron site | SSRF |
| Beamline | BL18U1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2021-01-06 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 0.9793 |
| Spacegroup name | P 31 |
| Unit cell lengths | 131.755, 131.755, 90.713 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 25.950 - 3.100 |
| R-factor | 0.2171 |
| Rwork | 0.215 |
| R-free | 0.24850 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 6cnr |
| RMSD bond length | 0.002 |
| RMSD bond angle | 0.592 |
| Data reduction software | HKL-3000 |
| Data scaling software | HKL-3000 |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.15.2_3472) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 35.180 | 3.150 |
| High resolution limit [Å] | 3.100 | 3.100 |
| Rmerge | 0.199 | |
| Rpim | 0.066 | 0.595 |
| Number of reflections | 31825 | 1606 |
| <I/σ(I)> | 18.1 | |
| Completeness [%] | 100.0 | |
| Redundancy | 10.3 | |
| CC(1/2) | 0.736 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 293 | 4.0 M sodium formate |
