7VST

Phosphoglucomutase_tlr1976

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL18U1
Synchrotron site	SSRF
Beamline	BL18U1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2020-11-02
Detector	DECTRIS PILATUS3 6M
Wavelength(s)	0.97915
Spacegroup name	C 2 2 21
Unit cell lengths	155.692, 155.724, 177.427
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	19.730 - 2.740
R-factor	0.2002
Rwork	0.199
R-free	0.22380
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1kfq
Refinement software	PHENIX (1.18.2_3874)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	19.730	2.820
High resolution limit [Å]	2.740	2.740
Rmerge	0.062	0.955
Number of reflections	56641	4606
<I/σ(I)>	17.4
Completeness [%]	99.7
Redundancy	6.8

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		298	NaCl