7VSQ

Crystal strcuture of the tandem B-Box domains of CONSTANS

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL19U1
Synchrotron site	SSRF
Beamline	BL19U1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2020-12-23
Detector	DECTRIS PILATUS3 6M
Wavelength(s)	0.9792
Spacegroup name	P 32
Unit cell lengths	50.813, 50.813, 97.624
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	32.684 - 2.700
R-factor	0.2787
Rwork	0.277
R-free	0.30480
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	7vsp
RMSD bond length	0.011
RMSD bond angle	1.762
Data reduction software	HKL-3000
Data scaling software	HKL-3000
Phasing software	PHENIX
Refinement software	PHENIX (1.9_1692)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	2.750
High resolution limit [Å]	2.700	2.700
Rmerge	0.168	1.053
Number of reflections	7733	386
<I/σ(I)>	13.3	1.2
Completeness [%]	99.5
Redundancy	9.4
CC(1/2)	0.996	0.867

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	5.6	293	0.2M ammonium acetate, 0.1M sodium citrate tribasic dehydrate, pH 5.6, 30% v/v (+/-)-2-Methyl-2,4-pentanediol