7VSP
Crystal strcuture of the tandem B-Box domains of COL2
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SSRF BEAMLINE BL19U1 |
Synchrotron site | SSRF |
Beamline | BL19U1 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2020-05-04 |
Detector | DECTRIS PILATUS3 6M |
Wavelength(s) | 0.9792 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 36.934, 44.478, 99.765 |
Unit cell angles | 90.00, 100.05, 90.00 |
Refinement procedure
Resolution | 22.930 - 2.100 |
R-factor | 0.1799 |
Rwork | 0.179 |
R-free | 0.20020 |
Structure solution method | SAD |
RMSD bond length | 0.012 |
RMSD bond angle | 1.230 |
Data reduction software | HKL-3000 |
Data scaling software | HKL-2000 |
Phasing software | PHENIX |
Refinement software | PHENIX (1.19.2_4158) |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 50.000 | 50.000 | 2.180 |
High resolution limit [Å] | 2.100 | 4.520 | 2.100 |
Rmerge | 0.089 | 0.052 | 0.340 |
Rmeas | 0.105 | 0.061 | 0.402 |
Rpim | 0.056 | 0.033 | 0.213 |
Total number of observations | 124258 | ||
Number of reflections | 18763 | 3595 | 3629 |
<I/σ(I)> | 4.2 | ||
Completeness [%] | 99.8 | 99.6 | 99.6 |
Redundancy | 3.4 | 3.5 | 3.4 |
CC(1/2) | 0.994 | 0.921 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 293 | 35% 1,4-Dioxane |