7VSJ
Crystal structure of MmIMP3-RRM12 in complex with 9-mer RNA
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SSRF BEAMLINE BL17B1 |
Synchrotron site | SSRF |
Beamline | BL17B1 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2021-10-23 |
Detector | DECTRIS PILATUS3 2M |
Wavelength(s) | 0.97853 |
Spacegroup name | P 2 21 21 |
Unit cell lengths | 39.811, 62.679, 72.663 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 28.780 - 2.380 |
R-factor | 0.2058 |
Rwork | 0.203 |
R-free | 0.26190 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 7EYY |
RMSD bond length | 0.008 |
RMSD bond angle | 1.004 |
Data reduction software | HKL-3000 |
Data scaling software | HKL-3000 |
Phasing software | PHASER |
Refinement software | PHENIX (1.19.2_4158) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 30.000 | 2.470 |
High resolution limit [Å] | 2.380 | 2.380 |
Rmerge | 0.146 | 0.731 |
Rmeas | 0.153 | 0.799 |
Rpim | 0.046 | 0.313 |
Number of reflections | 7733 | 763 |
<I/σ(I)> | 18.67 | 2 |
Completeness [%] | 99.9 | 99.5 |
Redundancy | 10.3 | 6 |
CC(1/2) | 0.993 | 0.754 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 5.6 | 293 | 0.2 M Ammonium acetate, 0.1 M Sodium citrate tribasic dihydrate pH 5.6, 30% w/v Polyethylene glycol 4000 |