7VSB

E. coli Ribonuclease HI in complex with one Zn2+ (His124 N-delta binding)

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SPRING-8 BEAMLINE BL45XU
Synchrotron site	SPring-8
Beamline	BL45XU
Temperature [K]	95
Detector technology	PIXEL
Collection date	2021-04-07
Detector	DECTRIS PILATUS3 6M
Wavelength(s)	1
Spacegroup name	P 43 21 2
Unit cell lengths	86.174, 86.174, 80.112
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	48.500 - 1.840
R-factor	0.1865
Rwork	0.185
R-free	0.22140
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4z0u
RMSD bond length	0.006
RMSD bond angle	0.746
Data reduction software	XDS
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	PHENIX (1.18.2_3874)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	48.500	1.880
High resolution limit [Å]	1.840	1.840
Rmerge	0.094	0.384
Rmeas	0.102	0.424
Rpim	0.041	0.179
Number of reflections	26808	1623
<I/σ(I)>	17.1
Completeness [%]	99.9
Redundancy	10.9
CC(1/2)	0.996	0.991

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	5.6	293	0.1 M sodium citrate tribasic dihydrate pH 5.6, 20% v/v 2-propanol, 20% w/v polyethylene glycol 4,000