7VS9
Crystal structure of P domain from norovirus GI.9 capsid protein in complex with Lewis x antigen.
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU FR-E SUPERBRIGHT |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2019-02-27 |
| Detector | RIGAKU RAXIS IV++ |
| Wavelength(s) | 1.5418 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 184.200, 77.244, 103.526 |
| Unit cell angles | 90.00, 124.20, 90.00 |
Refinement procedure
| Resolution | 35.207 - 2.260 |
| R-factor | 0.1661 |
| Rwork | 0.165 |
| R-free | 0.19450 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 7vp0 |
| RMSD bond length | 0.008 |
| RMSD bond angle | 0.931 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER |
| Refinement software | PHENIX ((1.13_2998: ???)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.340 |
| High resolution limit [Å] | 2.260 | 2.260 |
| Rmerge | 0.088 | 0.349 |
| Rmeas | 0.103 | 0.411 |
| Number of reflections | 56256 | 5539 |
| <I/σ(I)> | 15.3 | |
| Completeness [%] | 99.6 | |
| Redundancy | 3.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 9 | 298 | magnesium chloride, Bicine, pH 9.0 |
