7VS8

Crystal structure of P domain from norovirus GI.9 capsid protein in complex with Lewis b antigen.

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU FR-E SUPERBRIGHT
Temperature [K]	100
Detector technology	IMAGE PLATE
Collection date	2019-02-20
Detector	RIGAKU RAXIS IV++
Wavelength(s)	1.5418
Spacegroup name	C 1 2 1
Unit cell lengths	183.749, 76.464, 103.281
Unit cell angles	90.00, 124.20, 90.00

Refinement procedure

Resolution	41.193 - 2.400
R-factor	0.1663
Rwork	0.165
R-free	0.19560
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	7vp0
RMSD bond length	0.008
RMSD bond angle	0.973
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHASES
Refinement software	PHENIX ((1.13_2998: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	2.490
High resolution limit [Å]	2.400	2.400
Rmerge	0.073	0.333
Rmeas	0.086	0.395
Number of reflections	45674	4263
<I/σ(I)>	18.9
Completeness [%]	98.2
Redundancy	3.7

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	9	298	magnesium chloride, Bicine, pH 9.0