7VS7
Crystal structure of the ectodomain of OsCERK1 in complex with chitin hexamer
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRF BEAMLINE BL17U |
| Synchrotron site | SSRF |
| Beamline | BL17U |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2018-05-01 |
| Detector | AGILENT EOS CCD |
| Wavelength(s) | 1.0 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 46.043, 61.406, 46.409 |
| Unit cell angles | 90.00, 113.65, 90.00 |
Refinement procedure
| Resolution | 42.175 - 2.015 |
| R-factor | 0.1922 |
| Rwork | 0.190 |
| R-free | 0.23480 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4ebz |
| RMSD bond length | 0.006 |
| RMSD bond angle | 0.953 |
| Data reduction software | HKL-2000 |
| Data scaling software | SCALA |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.10-2155) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.070 |
| High resolution limit [Å] | 2.000 | 2.000 |
| Rmerge | 0.070 | 0.319 |
| Number of reflections | 15362 | 1486 |
| <I/σ(I)> | 25.78 | |
| Completeness [%] | 98.3 | |
| Redundancy | 5.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 293 | 0.25M Magnesium chloride, 0.1 M TRIS, pH8.5, and 30% (w/v) PEG4000. |
