7VRX
Pad-1 in the absence of substrate
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | PAL/PLS BEAMLINE 7A (6B, 6C1) |
| Synchrotron site | PAL/PLS |
| Beamline | 7A (6B, 6C1) |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2020-11-12 |
| Detector | ADSC QUANTUM 270 |
| Wavelength(s) | 0.97933 |
| Spacegroup name | P 65 |
| Unit cell lengths | 96.157, 96.157, 407.132 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 33.856 - 1.966 |
| R-factor | 0.253754535002 |
| Rwork | 0.253 |
| R-free | 0.29055 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1o4s |
| RMSD bond length | 0.006 |
| RMSD bond angle | 1.110 |
| Data reduction software | HKL-2000 (706e) |
| Data scaling software | HKL-2000 (706e) |
| Phasing software | PHASER (1.9_1692) |
| Refinement software | PHENIX (1.9_1692) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.040 |
| High resolution limit [Å] | 1.960 | 1.970 |
| Number of reflections | 149234 | 14983 |
| <I/σ(I)> | 18.705 | |
| Completeness [%] | 99.3 | |
| Redundancy | 10.7 | |
| CC(1/2) | 0.990 | 0.396 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 295 | 0.1 M sodium acetate (pH 4.6), 1.8 M ammonium sulfate |
