7VRG
Crystal structure of chitinase-h from O. furnacalis in complex with Lynamicin B
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | NFPSS BEAMLINE BL19U1 |
Synchrotron site | NFPSS |
Beamline | BL19U1 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2021-10-04 |
Detector | ADSC QUANTUM 315r |
Wavelength(s) | 0.97852 |
Spacegroup name | P 1 |
Unit cell lengths | 70.908, 77.859, 81.972 |
Unit cell angles | 113.63, 109.90, 95.00 |
Refinement procedure
Resolution | 43.030 - 2.400 |
R-factor | 0.2345 |
Rwork | 0.234 |
R-free | 0.24330 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 5gqb |
RMSD bond length | 0.008 |
RMSD bond angle | 0.936 |
Data reduction software | XDS |
Data scaling software | XSCALE |
Phasing software | PHASER |
Refinement software | PHENIX (1.17.1_3660) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 43.034 | 2.460 |
High resolution limit [Å] | 2.400 | 2.400 |
Number of reflections | 108457 | 7985 |
<I/σ(I)> | 4.64 | 0.88 |
Completeness [%] | 94.6 | 94.2 |
Redundancy | 3.5 | 3.6 |
CC(1/2) | 0.978 | 0.382 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 7 | 277 | 100mM HEPES pH 7.0, 26% (w/v) Jeffamine ED-2001 |