7VRF

Crystal structure of Oxpecker chromodomain in complex with H3K9me3

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL18U1
Synchrotron site	SSRF
Beamline	BL18U1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2016-07-07
Detector	DECTRIS PILATUS 6M
Wavelength(s)	0.9786
Spacegroup name	P 1
Unit cell lengths	31.421, 37.455, 38.757
Unit cell angles	61.23, 88.79, 74.94

Refinement procedure

Resolution	25.720 - 1.700
R-factor	0.1965
Rwork	0.192
R-free	0.23840
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4u68
RMSD bond length	0.012
RMSD bond angle	1.298
Data scaling software	HKL-3000
Phasing software	PHENIX
Refinement software	PHENIX (1.18.2_3874)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	30.000	1.730
High resolution limit [Å]	1.700	1.700
Rmerge	0.070	0.233
Number of reflections	15565	752
<I/σ(I)>	30.8
Completeness [%]	95.6
Redundancy	6.8

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		290	0.2M ammonium citrate, 20% (w/v) PEG 3350