7VQ6
Structure of a specialized glyoxalase from Gossypium hirsutum
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | LIQUID ANODE |
| Source details | BRUKER METALJET |
| Temperature [K] | 298 |
| Detector technology | CMOS |
| Collection date | 2020-09-21 |
| Detector | BRUKER PHOTON 100 |
| Wavelength(s) | 1.34 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 58.255, 49.507, 58.413 |
| Unit cell angles | 90.00, 99.38, 90.00 |
Refinement procedure
| Resolution | 44.480 - 1.390 |
| R-factor | 0.159 |
| Rwork | 0.157 |
| R-free | 0.18600 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1qip |
| RMSD bond length | 0.016 |
| RMSD bond angle | 2.048 |
| Data reduction software | SAINT |
| Data scaling software | SADABS |
| Phasing software | REFMAC |
| Refinement software | REFMAC (5.8.0238) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 44.480 | 1.490 |
| High resolution limit [Å] | 1.390 | 1.390 |
| Rmerge | 0.081 | 0.510 |
| Number of reflections | 66039 | 2731 |
| <I/σ(I)> | 12.8 | 2.09 |
| Completeness [%] | 99.8 | |
| Redundancy | 6.85 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 298 | MgAC; Sodium Cacodylate ; PEG 4000. |
