7VPT

Structure of the C. glabrata importin alpha ARM domain - Upc2 NLS fusion

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PAL/PLS BEAMLINE 7A (6B, 6C1)
Synchrotron site	PAL/PLS
Beamline	7A (6B, 6C1)
Temperature [K]	110
Detector technology	CCD
Collection date	2020-08-04
Detector	ADSC QUANTUM 270
Wavelength(s)	0.97934
Spacegroup name	P 63
Unit cell lengths	119.025, 119.025, 74.511
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	34.530 - 2.500
R-factor	0.2061
Rwork	0.200
R-free	0.26610
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4xgr
RMSD bond length	0.009
RMSD bond angle	1.123
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHASER
Refinement software	PHENIX (1.15.2_3472)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	2.540
High resolution limit [Å]	2.500	2.500
Rmerge	0.112	0.424
Number of reflections	20974	1048
<I/σ(I)>	46.4	12
Completeness [%]	99.9
Redundancy	11.7	12.2

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	8	296	0.1 M Tris-HCl pH 8.0, 30% PEG 400