7VPS

Crystal structure of the ARM domain of C. glabrata importin alpha

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PAL/PLS BEAMLINE 7A (6B, 6C1)
Synchrotron site	PAL/PLS
Beamline	7A (6B, 6C1)
Temperature [K]	110
Detector technology	CCD
Collection date	2020-08-04
Detector	ADSC QUANTUM 270
Wavelength(s)	0.97949
Spacegroup name	P 1 21 1
Unit cell lengths	47.650, 62.628, 154.646
Unit cell angles	90.00, 90.02, 90.00

Refinement procedure

Resolution	31.310 - 2.290
R-factor	0.2201
Rwork	0.218
R-free	0.26650
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4xgr
RMSD bond length	0.012
RMSD bond angle	1.594
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHASER
Refinement software	PHENIX (1.15.2_3472)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	2.340
High resolution limit [Å]	2.290	2.300
Rmerge	0.116	0.540
Number of reflections	39813	1934
<I/σ(I)>	14.5	3.2
Completeness [%]	97.0	95.9
Redundancy	2.8	2.6

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7	296	0.1 M Tris-HCl pH 7.0, 30% PEG 8000, 0.4 M NaCl