7VPF
Crystal structure of a novel putative sugar isomerase from the psychrophilic bacterium Paenibacillus sp. R4
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | PAL/PLS BEAMLINE 5C (4A) |
Synchrotron site | PAL/PLS |
Beamline | 5C (4A) |
Temperature [K] | 95 |
Detector technology | CCD |
Collection date | 2018-11-02 |
Detector | ADSC QUANTUM 315r |
Wavelength(s) | 0.97940 |
Spacegroup name | H 3 |
Unit cell lengths | 141.932, 141.932, 76.701 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 26.823 - 2.983 |
R-factor | 0.2167 |
Rwork | 0.214 |
R-free | 0.27120 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 3qc0 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | MOLREP |
Refinement software | PHENIX (1.14_3260) |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 50.000 | 10.000 | 3.050 |
High resolution limit [Å] | 2.980 | 7.340 | 2.980 |
Rmerge | 0.112 | 0.097 | 0.375 |
Rmeas | 0.118 | 0.102 | 0.394 |
Rpim | 0.036 | 0.033 | 0.122 |
Total number of observations | 117028 | ||
Number of reflections | 11523 | 639 | 759 |
<I/σ(I)> | 34.4 | ||
Completeness [%] | 98.3 | 81.4 | 99.7 |
Redundancy | 10.2 | 10.1 | 10.1 |
CC(1/2) | 0.989 | 0.962 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 293 | 0.2 M Sodium Fluoride, 0.1 M Bis-Tris propane (pH 7.5), 20% PEG 3350 |