7VPC
Neryl diphosphate synthase from Solanum lycopersicum
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SPRING-8 BEAMLINE BL32XU |
Synchrotron site | SPring-8 |
Beamline | BL32XU |
Temperature [K] | 80 |
Detector technology | PIXEL |
Collection date | 2021-07-28 |
Detector | DECTRIS EIGER X 9M |
Wavelength(s) | 1.0 |
Spacegroup name | C 1 2 1 |
Unit cell lengths | 98.643, 49.464, 119.494 |
Unit cell angles | 90.00, 91.21, 90.00 |
Refinement procedure
Resolution | 49.360 - 1.940 |
R-factor | 0.20298 |
Rwork | 0.200 |
R-free | 0.25502 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 5hxt |
RMSD bond length | 0.008 |
RMSD bond angle | 1.509 |
Data reduction software | DIALS |
Data scaling software | XSCALE |
Phasing software | MOLREP |
Refinement software | REFMAC (5.8.0258) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 2.050 |
High resolution limit [Å] | 1.940 | 1.940 |
Rmerge | 0.320 | 1.405 |
Rmeas | 0.351 | 1.556 |
Rpim | 0.205 | 0.929 |
Number of reflections | 83643 | 13527 |
<I/σ(I)> | 5.61 | 1.07 |
Completeness [%] | 99.3 | 98.9 |
Redundancy | 3.16 | 2.99 |
CC(1/2) | 0.929 | 0.523 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 7 | 293 | PEG 3350, DL-malic acid |